Job descriptions


Chemical safety
  • Ensure proper labeling of waste.
  • Get waste stickers from stockroom.
  • Insure proper placement of waste (hood in 104, 121).
  • Request waste pickup if required.
  • Interact with Paula Labbie from OEHS. Any questions should go to her.
  • Get rid of empty 4L bottles, or nag others to do so (dumpster).
Computers (SGI, PC)
  • Create new user accounts for paris mail server.
  • Install new software on Paris and Milan and additional SHIs.
  • Install patches/updates for IRIX and applications as necessary.
  • Printer responsibilities:
    1. Order supplies (Inks, toner cartridges, paper, transparencies)
    2. Renew service contracts
    3. Call for maintainance when necessary
Computers (Mac)
  • Maintain, update, and manage Macs in the computer room and throughout the lab.
  • Fix bugs as they happen and/or call for service.
  • Order new software/hardware as needs arise.
  • Keep software uniform and up to date on all Macs.
  • Keep hard drives clean and backed up.
  • Keep the lab informed on best practices regarding the Macs.

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Digital camera/Storm:
  • Call for maintenance/repairs
  • Train new members
FPLC
  • Keep watch over the three components to our system: 1. the FPLC itself (in delicase); 2. the fraction collector (also in delicase); and 3. a Flatbed Recorder (located on top of the incubator next to the delicase).
  • All instruction manuals are located in drawers labeled “FPLC” under the
    stopped-flow in 121. This drawer also contains various spare parts and tubing. Most columns are located in the delicase, but be aware that many haven't been used in years. There are also a few empty columns and accessories located under Anja's bench in KCL110.
  • We don't have a service contract, but any problems or questions should be
    directed to Amersham Pharmacia Biotech at 1-800-526-3593.

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HPLC
  • Maintain scheduling sheets
  • Liason to Agilent Engineer (Rick Rudolph, rick.rudolph@agilent.com; 413-427-9083)
  • Train users as necessary
  • Fix minor problems (re-prime pumps, reboot computers, fix leaks by tightening nuts/replacing ferrules/replacing lines).
  • Troubleshoot problems while in contact with Varian. (Sometimes it is much faster to install a replacement part supplied by Varian rather than wait for Engineers to get to Yale … we are under contract, and parts will be free).
  • Monitor CH3CN/H2O/TFA stocks. Place on shopping list as needed.
  • Monitor waste; must be labeled properly.
  • Nag people, re: neatness, sample filtration, waste labeling, scheduling, TURNING LAMPS OFF.
  • Clean columns w/isopropanol as needed (high column pressure, poor resolution).
Lyophilizer
  • Monitor ice levels in trap; defrost as necessary
  • Change oil once/year
  • Manage usage (nag users re: label samples, remove finished samples, clean messes); other groups may use only if there are available spots (with expected Schepartz usage in mind).
  • Contact Labconco with problems.

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PTI
  • Schedule maintenance under service contract
  • Train new group members
  • Purchase/maintain cuvettes
  • Maintain sign-in log
Radiation Safety Czar
  • Exchange badges quarterly
  • Discontinue badge service when members of the group leave
  • Fill out quarterly usage survey
  • See that new members receive appropriate Yale training
    (http://www.yale.edu/oehs/trainreq.htm for class schedule)
  • Train new members on weekly surveys, radioactive isotope ordering &
    check-in, wipe tests, use of scintillation counter, safe radioactive
    isotope usage
  • Maintain stocks vials (in particular, package and call for pickup when a
    significant number of non-empty stock vials remain that are no longer
    being used)
  • Repair survey meters as necessary
Stopped-flow
  • Direct inquiries to Ann Valentine

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Storm
  • The Storm is a model 840, serial number 90667. Tony Mendes is the Field Service Engineer. His card is taped to the instrument. Call
  • Amersham Biosciences for service (800-526-3593, option 3).
  • The Image Eraser is a model 810-UNV, serial number 84875. It's a big
    light box.
  • Supplies are plentiful, but order more casettes and storage phosphor
    screens if necessary.
  • The computer (pompeii.chem.yale.edu) can be tricky sometimes. When
    training new users, tell them to use ImageQuant 5.2 and back up and
    delete their files on the E:/ drive regularly.
Synthesizer
  • Guide users as to procedures … Do not synthesize peptides for people, but guide them closely as needed (which is very closely in the beginning).
  • Perform instrument cleaning periodically (instruct users how to perform routine cleaning after each synthesis)
  • Monitor usage/expected usage and schedule syntheses for people to maximize efficiency of instrument use.
  • Monitor Solvent 1 and N2 levels (replenish as needed).
  • Monitor amino acid, resin, and reagent stockpiles and purchase as needed.
  • Monitor Drierite in amino acid dessicator (under balance in 108). Replace as necessary.
  • Manage waste containers (siphon waste into disposal containers when necessary).
  • Manage reagent wastes in hood, and “stench” solid waste in hood. Solid waste is not turned over to OEHS; we remove “stenchy” solid waste containers to outside dumpsters ourselves (therefore only glass waste, paper waste, and gloves go in these boxes … no actual chemical waste). Janitors should not handle these boxes.
  • Contact Peter Bergwall with problems (business card on side of instrument).

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Cell culture
Incubators
Pfast System
Plasmid Czar
Refrigerators
Shakers
Shopping
Speed-vacs