Guidelines for FPLC use

  1. You must sign up when you use the FPLC. Note your name, the days on which you used the instrument, what you purified, what column you used, and check that you’ve cleaned everything to the best of your ability.
  2. Columns must be cleaned after use with how much a high salt buffer (what), and many are then stored in a 20% aqueous EtOH solution.
  3. Always detach and properly cap your column after use!
  4. Pumps, lines, etc. should be washed with how much water and how much 20% EtOH.
  5. Syringes and loops should be washed and stored (where).
  6. Injector port should be washed with how much of what.
  7. Most importantly, THERE SHOULD BE NO TRACE OF YOUR PROTEIN OR ANY OF WHAT YOU PURIFIED IT FROM LEFT IN THE FPLC AFTER YOU ARE FINISHED!!
  8. Pick up all of your pipette tips, eppys, etc.

THE FPLC IS FOR ALL OF US. If you are in doubt about how to clean it, please ask someone with more experience for help!

Thanks everyone for your help.